General 5-Hydroxytryptamine (5-HT) ELISA Kit

SKU: orb1806508

Description

General 5-Hydroxytryptamine (5-HT) ELISA Kit

Research Area

Gastroenterology & Hepatology, Infectious Disease & Virology, Neuroscience, Signal Transduction

Images & Validation

• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxytryptamine(5-HT) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxytryptamine(5-HT). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine(5-HT) in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: All
• Sample Types: serum, plasma, tissue homogenates
• Assay Type: Competitive
• Assay Time: 1.5H
• Range: 14.07-900ng/mL
• Sensitivity: 3.97 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5HT; Serotonin; N-Methyl-Gamma

Quick Database Links

Gene Symbol

5-HT