GAS7 Antibody (C-term)

SKU: orb1929916

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology, Cell Biology

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB20062
• Target: This GAS7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-465 amino acids from the C-terminal region of human GAS7.
• Molecular Weight: 54371 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Growth arrest-specific protein 7, GAS-7, GAS7, KIAA0394

GAS7 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with GAS7 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of GAS7 antibody (C-term) in A2058 cell line lysates (35 ug/lane). GAS7 (arrow) was detected using the purified Pab.

Western blot analysis of GAS7 antibody (C-term) in mouse cerebellum tissue lysates (35 ug/lane). GAS7 (arrow) was detected using the purified Pab.

Anti-GAS7 Antibody (C-term) at 1:1000 dilution + human cerebellum lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This GAS7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-465 amino acids from the C-terminal region of human GAS7.

UniProt

O60861

RefSeq (Protein):NP_958836.1, NP_001124303.1, NP_003635.2, NP_958839.1