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GAPDH Antibody for WB, IHC, IF & ELISA | Loading Control & PTM Studies
Reliable, multifunctional GAPDH Antibody for WB, IHC, IF, ELISA, and Flow Cytometry
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a widely used reference protein in protein expression studies, most commonly employed as a loading control in Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), ELISA, and other quantitative experiments.
Biorbyt provides a portfolio of polyclonal, monoclonal, and recombinant GAPDH Antibody, validated for Human, Mouse, Rat, and other species. These GAPDH Antibody products support diverse research areas, including metabolism, cancer, neuroscience, and neurodegenerative diseases.
What is GAPDH and Why It Matters in Research
- Nuclear transport and transcriptional regulation
- Apoptosis and oxidative stress response
- Post-translational modifications (PTMs) such as phosphorylation and S-nitrosylation
- Cellular signaling pathways including PI3K/AKT, MAPK, and apoptosis-related cascades
Its ubiquitous and stable expression makes GAPDH a reliable loading control, while certain GAPDH Antibody products also enable functional studies, PTM detection, and protein-protein interaction analyses.
Recommended GAPDH Antibody Products and Research Applications
SKU | Product Name | Applications | Size | Price | Research Focus / Mechanism |
|---|---|---|---|---|---|
orb500826 | GAPDH Rabbit Polyclonal Antibody – High Sensitivity Loading Control (Literature-Supported) | WB, IHC-P, IHC-Fr, IF, ELISA | 100 μl | $220 | Energy metabolism, glycolysis, PI3K/AKT signaling |
orb704534 | GAPDH Recombinant Rabbit Monoclonal Antibody – Quantitative & Reproducible | WB, IHC-P, IHC-Fr, IF, FC,ICC | 50 μl | $160 | Cancer research, MAPK, apoptosis pathways |
orb1928806 | GAPDH Antibody (N-terminal) – Functional Localization Studies (Literature-Supported) | WB, IF, IHC-P | 50 μl | $170 | Nuclear GAPDH, transcription regulation, oxidative stress |
orb1928805 | GAPDH Antibody (C-terminal R248) – PTM & Flow Cytometry Compatible | WB, IF, IHC-P, FC | 50 μl | $170 | PTM detection, intracellular signaling, co-staining |
orb234217 | WB, IHC | 100 μl | $420 | PTM and functional studies |
Why Choose Biorbyt GAPDH Antibody?
- High specificity and sensitivity across multiple species including Human, Mouse, Rat, and non-mammalian models
- Polyclonal, monoclonal, and recombinant formats to match experimental needs and reproducibility requirements
- PTM validated antibodies for phosphorylation, acetylation, and S-nitrosylation detection
- Literature-supported options to ensure reliability in published workflows
- Cross-application validation: WB, IHC, IF, ELISA, Flow Cytometry, Dot, and IP
- Consistent quality and lot-to-lot reproducibility, ideal for long-term projects
- Expert scientific support and troubleshooting, ensuring confidence in experimental results
How to Choose the Best GAPDH Antibody for Your Research
- Application Type: WB, IHC, IF, ELISA, FC, Dot, IP
- Epitope Location:
- N-terminal GAPDH Antibody: nuclear localization, full-length detection
- C-terminal GAPDH Antibody: PTM detection, flow cytometry, Dot assays
- Antibody Format:
- Polyclonal: broad epitope recognition, high sensitivity
- Recombinant monoclonal: reproducible and quantitative
- Species Reactivity: Human, Mouse, Rat, Yeast, Zebrafish
- Functional Studies: PTM detection, protein interactions, nuclear/cytoplasmic localization
- Literature Validation: check prior studies for reproducibility
Applications and Functional Studies of GAPDH Antibody
- WB: normalization, PTM detection
- IHC-P / IHC-Fr: tissue localization and quantification
- IF / ICC: subcellular distribution, PTM detection
- FC: intracellular detection
- ELISA / Dot assays: quantitative analysis
- IP: study protein interactions and PTMs
FAQ
What is the difference between N-terminal and C-terminal GAPDH Antibody?
- N-terminal: nuclear localization, full-length detection
- C-terminal: PTM detection, flow cytometry, Dot assays
Can non-loading control GAPDH Antibody be used as a loading control?
Yes, if it recognizes full-length GAPDH and shows stable, linear expression. Validation in your system is recommended.
When should I choose GAPDH as a loading control, and when should I use an alternative like β-actin?
- Use GAPDH when its expression is stable across samples and treatments for WB, IHC, IF, or ELISA normalization.
- Use alternatives such as β-actin, α-tubulin, or HSP90 when GAPDH may fluctuate due to metabolic stress, hypoxia, differentiation, or treatments affecting glycolysis.
- For PTM or functional studies, select based on antibody specificity and experimental validation.
Conclusion
Biorbyt GAPDH Antibody provides high specificity, multi-application validation, and cross-species coverage, supporting both classical loading control and functional/PTM studies. Researchers can select the optimal GAPDH Antibody based on epitope, application, and species to achieve accurate, reproducible, and robust experimental results.