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GAD67 Antibody

Catalog Number: orb606596

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606596
CategoryAntibodies
DescriptionThis mAb recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1). GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.
Species/HostMouse
ClonalityMonoclonal
Clone NumberGAD1/2391
Tested applicationsELISA, IHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenA portion of amino acids 72-135 from the human protein were used as the immunogen for this GAD67 antibody.
Antibody TypePrimary Antibody
Dilution rangeELISA (order BSA/sodium azide-free format for coating),Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis GAD67 antibody is available for research use only.
UniProt IDQ99259
StorageStore the GAD67 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the GAD67 antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
GAD67 Antibody

Western blot testing of human brain lysate with GAD67 antibody. Expected molecular weight ~67 kDa.

GAD67 Antibody

IHC testing of FFPE human brain with GAD67 antibody (clone GAD1/2391). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

GAD67 Antibody

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using GAD67 antibody (clone GAD1/2391). These results demonstrate the foremost specificity of the GAD1/2391 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

GAD67 Antibody

SDS-PAGE analysis of purified, BSA-free GAD67 antibody (clone GAD1/2391) as confirmation of integrity and purity.

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