FST Antibody

SKU: orb1272758

Description

FST Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Human
• Application Notes:
  Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100.0 ng/mL), a concentration of 8.0-12.0 μg/mL of this antibody is required.ELISA:Indirect:To detect hFollistatin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin.SandwichTo detect hFollistatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Follistatin as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFollistatin. Western Blot:To detect hFollistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFollistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFollistatin. Human Follistatin specific antibody was purified by affinity chromatography employing immobilized hFollistatin matrix.
• Target: FST
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

FSFollistatin, Activin-binding protein, FS

To detect Human Follistatin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Follistatin (orb1272757) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Follistatin.

To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Follistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Follistatin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Quick Database Links

Gene Symbol

FST

UniProt

P19883

RefSeq:P19883