Flavoxate hydrochloride

SKU: orb1310369

Description

Flavoxate hydrochloride (DW61) is a muscarinic acetylcholine receptor antagonist employed as a research tool. It is utilized in studies of urinary tract disorders and smooth muscle spasm, with applications in both in vitro receptor binding assays and in vivo pharmacological models.

Research Area

Metabolism Research, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 3717-88-2
• MW: 427.92
• Purity: 99.78%
• Formula: C₂₄H₂₆ClNO₄
• SMILES: Cl.Cc1c(oc2c(cccc2C(=O)OCCN2CCCCC2)c1=O)c1ccccc1
• Target: PDE,Calcium Channel,AChR
• Solubility: DMSO:1 mg/mL (2.34 mM)

Bioactivity

• Target IC50:
  mAChR:12.2 μM
• In Vivo:
  Flavoxate inhibits cAMP formation in a concentration-dependent manner (0.01~10 μM) from membranes extracted from the rat cerebral cortex and striatum, an effect completely negated by pertussis toxin pretreatment. At concentrations greater than 10 μM, Flavoxate suppresses detrusor muscle contraction induced by acetylcholine, with a pD value of 4.55, and also inhibits contractions induced by Ca2+, with a pIC50 value of 4.92. Flavoxate concentration-dependently reduces K+-induced contractions of human bladder. It can displace [3H]nitrendipine at Ca2+ channel binding sites, with an IC50 of 254 μM. Flavoxate also concentration- and voltage-dependently inhibits the peak amplitude of inward Ba2+ currents sensitive to the voltage-dependent nifedipine in human detrusor smooth muscle cells (Ki: 10 μM), and inhibits the same Ba2+ currents at both 30°C (Ki = 5.1 mM) and 37°C (Ki = 4.6 mM).
• In Vitro:
  Without altering basal bladder pressure in rats, Flavoxate (3 mg/kg) can halt efferent nerve activity and associated bladder contractions for approximately 10 minutes. At the same dosage, administered intravenously, Flavoxate abolishes rhythmic bladder contractions, with maximum intervals of voiding contractions reaching 7.20 minutes. In rats, at a higher concentration (10 mg/kg), Flavoxate suppresses both the initial rapid-rise contraction phase (Phase 1) and the sustained contraction phase (Phase 2) to an equivalent degree. Furthermore, at this concentration, Flavoxate eliminates bladder contractions without altering contraction amplitude. Intracerebroventricular (ICV) injections of Flavoxate ranging from 50 to 200 mg or intrathecal (IT) injections of 100 to 200 mg dose-dependently abolish rhythmic bladder contractions within 5 to 15 minutes post-administration.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DW 61, DW61, DW-61, anesthetic, antispasmodic, Calcium Channel, Ca2+ channels, Ca channels, Inhibitor, Flavoxate, Flavoxate HCl, Flavoxate hydrochloride, Flavoxate Hydrochloride, mAChR, lower urinary tract infections, NSC114649, NSC-114649, overactive bladder, Muscarinic acetylcholine receptor, muscarinic, inhibit, NSC 114649, Phosphodiesterase (PDE), Phosphodiesterase, Rec-7-0040

Compound Identifiers

PubChem CID

441345