FITC-Dextran (MW 10000)

SKU: orb1307191

Description

FITC-Dextran (10 kDa) is a fluorescently labeled polysaccharide tracer used to assess vascular permeability, including that of the blood-brain barrier, in both in vivo and in vitro models. Its size-dependent diffusion makes it a key tool for studying solute transport and barrier integrity in neuroscience and vascular biology research.

Key Properties

• CAS Number: 60842-46-8
• MW: 10000
• Target: Others
• Solubility: DMSO:247.5 mg/mL (24.75 mM);H2O:50 mg/mL (5 mM)

Bioactivity

• In Vivo:
  METHODS: Intestinal permeability was determined by FITC-dextran: 1. FITC-dextran was dissolved in sterilized PBS at a concentration of 80 mg/mL. 2. Mice were fasted for 4 h prior to FITC-dextran injection, followed by oral administration of 100 µL of FITC-dextran. 3. After 4 h, mouse blood is collected and centrifuged at 4000 rpm for 15 min. Plasma samples are collected in clear EP tubes and placed at 4°C, protected from light. 4. Standard FITC-dextran samples were diluted to 0-100 µg/mL with PBS and transferred to a black 96-well plate. 5. Measure the fluorescence at 485 nm excitation wavelength and 528 nm emission wavelength using a microplate reader.
• In Vitro:
  METHODS: FITC-dextran assay for RBL-2H3 mast cell line: 1. Mix 1 mg of FITC-dextran powder per 1 mL of medium. Use a cellulose acetate syringe filter with a pore size of 0.22 µm to filter the dissolved FITC-dextran. 2. Add 10 µL of cell suspension to a chamber coverslip pre-filled with fresh FITC-dextran supplemented medium. 3. Incubate RBL cells overnight at 37°C in a humidified environment with 5% CO2. Cells should be maintained at a subfusion level to ensure cell separation and that each cell can be easily identified individually under a microscope. 4. Wash the chamber coverslips three times by aspirating the medium from the chamber and refilling with 300 µL of Tyrode Buffer preheated to 37°C. The coverslips should be cleaned with the Tyrode Buffer. Finally, replenish the chamber with 300 µL of Tyrode Buffer preheated to 37°C. 5. Select the region of interest to be tracked by turning on the fluorescent light source and selecting an appropriate fluorescent filter, choose a filter with green fluorophores to view FITC-dextran. if using a laser-based microscope, turn on the 488 nm laser, the emission should be centered around 500-550 nm.

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FITC-Dextran, FITCDextran (MW 10000), FITC Dextran (MW 10000)

Quick Database Links

Gene Symbol

Others