fibrinogen beta chain Antibody

SKU: orb1248226

Description

fibrinogen beta chain Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Liver lysates (calculated MW of 55.9kDa according to NP_005132.2). Recommended concentration: 0.01-0.03µg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Liver for this particular batch.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-EKHQLYIDETVNSN
• Target: fibrinogen beta chain
• Molecular Weight: Approx 60kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FGB, fibrinogen beta chain, fibrinogen, B beta polypeptide

orb1248226 (0.01 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248226 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Quick Database Links

Gene Symbol

fibrinogen beta chain

UniProt

P02675

RefSeq (Protein):NP_001171670.1, NP_005132.2