fibrinogen beta chain Antibody

• Catalog Number: orb1248226
• Category: Antibodies
• Description: fibrinogen beta chain Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-EKHQLYIDETVNSN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Liver lysates (calculated MW of 55.9kDa according to NP_005132.2). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Liver for this particular batch.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 60kDa
• Target: fibrinogen beta chain
• UniProt ID: P02675
• NCBI: NP_001171670.1, NP_005132.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FGB, fibrinogen beta chain, fibrinogen, B beta pol
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Liver lysates (calculated MW of 55.9kDa according to NP_005132.2). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Liver for this particular batch.Immunohistochemistry:Paraffin embedded Human Placenta and Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248226 (0.01 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248226 (5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.