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FER Antibody (C-term)
Description
Images & Validation
−| Tested Applications | FC, IF, IHC-P, WB |
|---|---|
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB03222 |
| Target | This FER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 782-811 amino acids from the C-terminal region of human FER. |
| Molecular Weight | 94638 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Disclaimer | For research use only |
Alternative Names
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The anti-FER Pab is used in Western blot to detect FER in HL-60 cell lysate.

FER Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of FER Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

FER Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FER Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_005237.2 |
|---|
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Protocol Information
FER Antibody (C-term) (orb1928962)
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