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Item 1 of 3
FEN1 Antibody (Center)
Catalog Number: orb1931392
| Catalog Number | orb1931392 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB17639 |
| Tested applications | IF, WB |
| Predicted Reactivity | Sheep |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:100, WB: 1:1000, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Conjugation | Unconjugated |
| MW | 42593 Da |
| Target | This FEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-272 amino acids from the Central region of human FEN1. |
| UniProt ID | P39748 |
| NCBI | NP_004102.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Flap endonuclease 1 {ECO:0000255|HAMAP-Rule:MF_031 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescent analysis of U251 cells, using FEN1 Antibody (Center). Diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of anti-FEN1 Antibody (Center) in HepG2 cell line lysates (35 ug/lane). FEN1 (arrow) was detected using the purified Pab.
Western blot analysis of FEN1 (arrow) using rabbit polyclonal FEN1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FEN1 gene.
