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F2R/Thrombin Receptor/PAR1 Antibody

SKU: orb1538216

Description

F2R/Thrombin Receptor/PAR1 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeIHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:64000), WB (0.3 - 1 µg/ml)
ReactivityHuman
Application Notes
Further information: Approx 48kDa band observed in lysates of cell line HeLa (calculated MW of 47.4kDa according to NP_001983.2).

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence C-TRARRPESKATNAT, from the Internal region (near N-Terminus) of the protein sequence according to NP_001983.2.
TargetF2R / Thrombin Receptor / PAR1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1

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F2R/Thrombin Receptor/PAR1 Antibody

F2R / Thrombin Receptor / PAR1 antibody (0.3 µg/ml) staining of HeLa lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

F2R/Thrombin Receptor/PAR1 Antibody (orb1538216)

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50 μg
$ 670.00
DispatchUsually dispatched within 2-3 weeks
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