EWSR1 Antibody

• Catalog Number: orb1262496
• Category: Antibodies
• Description: EWSR1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This EWSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 628-656 amino acids from the C-terminal region of human EWSR1.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: EWSR1
• UniProt ID: Q01844
• NCBI: Q01844
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: RNA-binding protein EWS, EWS oncogene, Ewing sarco
• Note: For research use only
• Application notes: For IF starting dilution is: 1:10~50For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of U251 cell stained with EWSR1 Antibody.U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly.

Western blot analysis of EWSR1 Antibody in A2058 cell line lysates (35 ug/lane)

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.