Eugenol

SKU: orb1309073

Description

Eugenol (Allylguaiacol) is a standardized allergen that acts through enhanced histamine release and cell-mediated immune responses. It is widely used in immunology and dermatology research to study allergic contact dermatitis mechanisms in both in vitro assays and in vivo models.

Research Area

Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 97-53-0
• MW: 164.20
• Purity: 99.72%
• Formula: C₁₀H₁₂O₂
• SMILES: O(C)C1=CC(CC=C)=CC=C1O
• Target: Reactive Oxygen Species,Apoptosis,Antibacterial,Ferroptosis,Parasite
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:5.00 mg/mL (30.45 mM);DMSO:240.00 mg/mL (1461.63 mM)

Bioactivity

• In Vivo:
  Eugenol inhibit the proliferation of breast cancer cells in vivo as well the inhibitory effect of eugenol on onco-proteins is also observed in vivo in tumor xenograft. Eugenol (0.2, 1.0, 5.0 or 25 mg/kg) when given orally at three different times in relation to the time of CCl4 dosing (i.p administration of 0.4 mg/kg), i.e. prior to (-1 hr), along with (0 hr) or after (+ 3 hrs), prevents significantl the rise in SGOT(Serum glutamic-oxaloacetic transaminase) activity, lipid peroxidation as well as liver necrosis the Protective effect is more evident at 1 mg and 5 mg eugenol doses than at 0.2 and 25 mg does. however the decrease in microsomal G-6-pase activity by CCl4 treatment is not prevented by eugenol suggesting tha the damage to endoplasmic reticulum is not protecte.
• In Vitro:
  Eugenol has ant Proliferative effects in diverse cancer cell lines as well as in B16 melanoma xenograft model. Eugenol induces apoptosis in various cancer cells, including mast cells, melanoma cells and HL-60 leukemia cells. Eugenol at low dose (2 μM) has specific toxicity against different breast cancer cells. This killing effect is mediated mainly through inducin the internal apoptotic pathway and strong down-regulation of E2F1 and its downstream antiapoptosis target survivin, independently o the status of p53 and ERα. Eugenol inhibits also several Other breast cancer related oncogenes, such as NF-κB and cyclin D1. Moreover, eugenol up-regulate the versatile cyclin-dependent kinase inhibitor p21WAF1 protein, and inhibit the proliferation of breast cancer cells in a p53-independent manne.
• Cell Research:
  Cells are seeded into 96-well plates and incubated overnight the medium is replaced with fresh one containin the desired concentration of eugenol. After 20 hrs, 10 μL o the WST-1 reagent is added to each well an the plates are incubated for 4 hrs at 37℃he amount of formazan is quantified using ELISA reader at 450 nM of absorbance.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Apoptosis, Antibacterial, Bacterial, 4-Allyl-2-methoxyphenol, 4-Allylguaiacol, Allylguaiacol, Parasite, inhibit, Ferroptosis, Eugenic acid, Eugenol, Inhibitor, ReactiveOxygenSpecies, Reactive Oxygen Species

Compound Identifiers

PubChem CID

3314