Ethyl 3,4-dihydroxybenzoate

SKU: orb1301586

Description

Ethyl 3,4-dihydroxybenzoate (EDHB) is a cell-permeable prolyl hydroxylase inhibitor used in hypoxia research. It has demonstrated efficacy in attenuating acute hypobaric hypoxia-induced vascular leakage in the brain in vivo, making it a valuable tool for studying HIF stabilization and related pathways.

Research Area

Cell Biology, Epigenetics & Chromatin, Immunology & Inflammation, Metabolism Research, Signal Transduction

Key Properties

• CAS Number: 3943-89-3
• MW: 182.17
• Purity: 99.88%
• Formula: C₉H₁₀O₄
• SMILES: CCOC(=O)C1=CC(O)=C(O)C=C1
• Target: Autophagy,HIF/HIF Prolyl-Hydroxylase,ROS,NO Synthase,Reactive Oxygen Species,Apoptosis
• Solubility: DMSO:22.5 mg/mL (123.51 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (10.98 mM)

Bioactivity

• In Vivo:
  EDHB supplementation effectively scaled down HH induced cerebral edema with concomitant downregulation of brain NF-κB expression. There was significant curtailment of pro-inflammatory cytokines and cell adhesion molecules. There was significant downregulation of permeability factor VEGF by EDHB with concomitant increment in hypoxia inducible factor (HIF1α) and anti-inflammatory proteins HO-1 and MT-1 compared to HH control thus accentuating the potential of EDHB as effective hypoxic preconditioning agent in ameliorating HH mediated injury in brain.
• In Vitro:
  It is shown that DHB reversibly inhibits both morphological and biochemical differentiation of myoblasts, if it is added to the culture medium before the cell alignment stage.Along with the inhibition of procollagen synthesis, DHB also decreases markedly the production of a collagen-binding glycoprotein (gp46) present in the ER.
• Animal Research:
  Male Sprague-Dawley rats treated with EDHB (75 mg/kg for 3 days), were subjected to acute HH exposure at 9144 m (30,000 ft) for 5 h. Animals were assessed for transvascular leakage and edema formation in brain and role of key inflammatory markers along with hypoxia responsive genes.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

prolyl hydroxylase, prolyl-hydroxylase, ROS, ReactiveOxygenSpecies, Reactive Oxygen Species, Hypoxia-inducible factors, Inhibitor, HIF, HIF Prolyl-Hydroxylase, HIF ProlylHydroxylase, HIF/HIF ProlylHydroxylase, HIF/HIF Prolyl-Hydroxylase, HIF/HIFProlylHydroxylase, HIF-PH, HIFProlylHydroxylase, HIFs, Ethyl protocatechuate, Ethyl 3,4 dihydroxybenzoate, Ethyl 3,4dihydroxybenzoate, Ethyl 3,4-dihydroxybenzoate, inhibit, NOSynthase, NOS, mitochondrial, NO Synthase, Nitric oxide synthases, collagen, antioxidant, Apoptosis, Autophagy

Compound Identifiers

PubChem CID

77547