ERBB2/HER2 Rabbit Polyclonal Antibody

SKU: orb10372

Description

ERBB2/HER2 Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HER2 (1201-1255/1255aa)
• Target: ERBB2
• Molecular Weight: 185 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD340; HER-2; HER-2/neu; HER2; MLN 19; MLN-19; NEU; NGL; TKR1; VSCN2; c-ERB-2; c-ERB2; p185(erbB2); Erbb-2; c-erbB2; c-neu; l11Jus8; mKIAA3023; HER2/neu; ERBB2_HUMAN; ERBB2; Metastatic lymph node gene 19 protein (MLN 19); Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; 2.7.10.1; MLN19; ERBB2_MOUSE; Kiaa3023; ERBB2_RAT; Epidermal growth factor receptor-related protein; p185neu;

Tissue/Cell: MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (HER2) polyclonal Antibody, Unconjugated (orb10372) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

ERBB2

UniProt

P04626