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Description
Research Area
Cardiovascular Research
Images & Validation
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| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized rhEPHB4 at 2μg/ml (100μl/well) can bind human EphrinB2 with a linear range of 0. 8 - 85 ng/mL. |
| Target | EPHB4 |
| Tag | His Tag |
| MW | 57.8 kDa |
| Purity | >97% as determined by SDS-PAGE. |
| Protein Sequence | Glu 17 - Ala 539 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EphB4, HTK, MYK1, TYRO11
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The purity of rh EPHB4 was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_004435.3 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
EPHB4 Recombinant Protein (orb1228074)
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