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| Catalog Number | orb764317 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to NOS3 (phospho-Ser1177) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser1176. AA range:1144-1193 |
| MW | 133 |
| Tested applications | ELISA, IF, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-NOS3 antibody, anti-Nitric oxide synthase ant Read more... |
| Note | For research use only |
Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min).3, Picture B: DAPI (blue) 10min.
Immunofluorescence analysis of Hela cell. 1, NOS3 (phospho Ser1177) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Western Blot analysis of various cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1:1000.
Western Blot analysis of HuvEc cells using Phospho-NOS3 (S1177) Polyclonal Antibody diluted at 1:1000.
Phospho-eNOS (Ser1177) Rabbit pAb [orb2715672]
WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl
