EME1 Antibody

SKU: orb2644522

Description

Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3 DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the EME1 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: MTA31 7H2/1
• Immunogen: His-tagged recombinant Eme1 of human origin was used as the immunogen for the EME1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

Q96AY2