Elastin antibody

SKU: orb2276289

Description

Elastin antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Colon: A moderate to strong elastin immunostaining should be seen of fibres in the lamina propria, around smooth muscle cells of the muscularis mucosae, and in blood vessels (subendothelial). Negative Control: Colon: Elastin immunostaining should be absent in all epithelial cells. Cellular Localization: Extracellular Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-648R
• Immunogen: Recombinant full-length human Elastin protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Elastin; ELN; SVAS; Tropoelastin; WBS; WS

Bronchus mucosa High content of elastin fibres along the bronchus mucosa.

Colon descendes mucosa Elastin fibres predominate in the lamina propria around smooth muscle cells of the muscularis mucosae and in blood vessels.

Testis High density of elastin fibres along smooth muscles surrounding tubuli of the testis.

Quick Database Links

UniProt

P15502