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Catalog Number | orb1258208 |
---|---|
Category | Antibodies |
Description | EGLN2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EGLN2 (NP_444274.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IP: 1:20 - 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 43kDa |
Target | EGLN2 |
UniProt ID | Q96KS0 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | EGLN2, EIT6, PHD1, HPH-1, HPH-3, HIFPH1, HIF-PH1 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IP: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using EGLN2 antibody (orb1258208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunoprecipitation analysis of 150 ug extracts of HeLa cells using 3 ug EGLN2 antibody (orb1258208). Western blot was performed from the immunoprecipitate using EGLN2 antibody (orb1258208) at a dilution of 1:1000.
ELISA, IF, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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