EGLN1 / PHD2 Antibody

• Catalog Number: orb639874
• Category: Antibodies
• Description: 366G/76/3 recognises human prolyl hydroxylase 2 (PHD2), a 46kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 366G/76/3
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Amino acids 1-24 of human PHD2/EGLN1 were used as the immunogen for the EGLN1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This EGLN1 / PHD2 antibody is available for research use only.
• UniProt ID: Q9GZT9
• Storage: Store the EGLN1 / PHD2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free EGLN1 antibody (clone 366G/76/3) as confirmation of integrity and purity.