EGFR rabbit pAb

• Catalog Number: orb765111
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EGFR
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human EGFR. AA range:836-885
• MW: 140
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-EGFR antibody, anti-ERBB antibody, anti-ERBB1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of AD293 cells using EGFR Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of various cells using EGFR Polyclonal Antibody diluted at 1:1000.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using EGFR Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A431 cells, treated with EGF 40μM 10', using EGFR Antibody. The lane on the right is blocked with the synthesized peptide.