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| Catalog Number | orb767930 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to EGFR (phospho-Tyr869) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr869. AA range:836-885 |
| MW | 175 |
| Tested applications | ELISA, IF, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-EGFR antibody, anti-ERBB antibody, anti-ERBB1 Read more... |
| Note | For research use only |
Western Blot analysis of 453 cells using Phospho-EGFR (Y869) Polyclonal Antibody diluted at 1:1000.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using EGFR (Phospho-Tyr869) Antibody.
Western blot analysis of lysates from A431 cells treated with EGF 40 muM 10', using EGFR (Phospho-Tyr869) Antibody. The lane on the left is blocked with the phospho peptide.
Phospho-EGFR (Tyr869) Rabbit pAb [orb1924789]
WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl, 20 μl
