DVL3 Antibody (C-term)

• Catalog Number: orb1937782
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This DVL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530-557 amino acids from the C-terminal region of human DVL3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q92997
• MW: 78055 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:10~50, WB: 1:2000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB31712
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Segment polarity protein dishevelled homolog DVL-3
• Research Area: Cardiovascular Research, Neuroscience, Signal Tran
• Note: For research use only
• NCBI: NP_004414.3

All lanes: Anti-DVL3 Antibody (C-term) at 1:2000 dilution. Lane 1: A549 whole cell lysates. Lane 2: NIH/3T3 whole cell lysates. Lane 3: K562 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 78 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of DVL3 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

DVL3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DVL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.