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Catalog Number | orb346950 |
---|---|
Category | Antibodies |
Description | Dog IgG F(c) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Canine |
Isotype | IgG |
Immunogen | Dog IgG F(c) fragment |
Concentration | 2.1 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Dog IgG, Dog IgG F(c) and Dog Serum. No reaction was observed against Dog IgG F(ab). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti Dog IgG F(c) Antibody, rabbit anti Dog Read more... |
Note | For research use only |
Application notes | Anti-Dog IgG F(c) antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
ELISA, IHC, SDS-PAGE, WB | |
Unconjugated | |
Dog IgG F(c) Fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Dog IgG F(c) Fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog Serum, anti-Dog IgG and anti-Dog IgG F(c). No reaction was observed against anti-Dog IgG F(ab’)2 or anti-Papain. | |
Dog |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Dog IgG, anti-Dog IgG F(c) and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(ab’)2 or anti-Papain. | |
Dog |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Dog IgG, anti-Dog IgG F(c) and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(ab’)2 or anti-Papain. | |
Dog |
Biotin | |
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Dog IgG, anti-Dog IgG F(c) and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(ab’)2 or anti-Papain. | |
Dog |
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