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Dog Clusterin (CLU) ELISA Kit

Dog Clusterin (CLU) ELISA Kit

Catalog Number: orb2667485

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667485
CategoryAssays and Kits
DescriptionDog Clusterin (CLU) ELISA Kit
Range1.57-100 µg/mL
Assay Time3.5h
UniProt IDP25473
Sensitivity0.48 µg/mL
Alternative namesCLU; TRPM2; KUB1; CLI; APOJ; SGP2; AAG4; APO-J; SP
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NoteFor research use only
Application notesstandard: 100 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog CLU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog CLU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog CLU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog CLU in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
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