DNA Damage (8-OHdG) ELISA Kit

• Catalog Number: orb1822520
• Category: Assays and Kits
• Description: Colorimetric detection of 8-hydroxy-2-deoxy Guanosine
• Target: DNA Damage (8-OHdG)
• Reactivity: All
• Application notes: 1. Prepare standard and samples in the Sample and Standard Diluent. 2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells. 3. Add 50 µL of the diluted antibody preparation to the appropriate wells. 4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour. 5. Wash plate 4 times with 1X Wash Buffer. 6. Add 100 µL of TMB Substrate to each well. 7. Cover plate and develop the plate in the dark at room temperature for 30 minutes. 8. Add 100 µL of Stop Solution to each well. 9. Measure absorbance on a plate reader at 450 nm. 10. Plot the standard curve and calculate sample concentrations.
• Cross Reactivity: Cross-reactivity: 8-Hydroxy-2-deoxy Guanosine (8-OHdG): 100%. 8-Hydroxy Guanosine (8-OHG): 23%. 8-Hydroxy Guanine (8-oxoG): 23%. Guanosine: < 0.01%.
• Assay Type: Competitive
• Range: 0.94 - 60 ng/mL
• Sensitivity: 0.59 ng/mL
• Sample Types: Urine, Cell Lysates, Plasma, Sample matrices
• Storage: 4°C and -20°C
• Alternative names: 8-OH-dG ELISA Kit, 8OHG ELISA Kit, 80G ELISA Kit,
• Note: For research use only

Typical Standard Curve for the DNA Damage (8-OHdG) ELISA kit (Enzyme-Linked Immunosorbent Assay). Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.94 - 60 ng/ml.

Chemical Equation of the Oxidation of Guanosine

Urine Spike Assay