Diphenyleneiodonium chloride

SKU: orb1299994

Description

Diphenyleneiodonium chloride (DPI) is an irreversible inhibitor of iNOS and eNOS with IC50 values of 50 nM and 0.3 μM, respectively. It also exhibits broad-spectrum bactericidal activity, making it a valuable tool for studying nitric oxide biology and microbial pathogenesis in both cellular and animal models.

Research Area

Immunology & Inflammation, Metabolism Research, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 4673-26-1
• MW: 314.55
• Purity: 99.86% (May vary between batches)
• Formula: C₁₂H₈ClI
• SMILES: [Cl-].[I+]1c2ccccc2-c2ccccc12
• Target: Reactive Oxygen Species,ROS,NOS,TRP/TRPV Channel,NADPH
• Solubility: DMSO:6 mg/mL (19.07 mM)

Bioactivity

• Target IC50:
  eNOS:0.3 µM|iNOS:50 nM
• In Vitro:
  Diphenyleneiodonium chloride (DPIC), an NADPH/NADH oxidase inhibitor, as possessing potent antimicrobial activity against non-tuberculous mycobacteria. DPIC exhibited concentration-dependent bactericidal activity against M. fortuitum and synergized with amikacin, ceftriaxone, ceftazidime and meropenem.
• Cell Research:
  Whole-cell growth inhibition assays were used to screen and identify novel inhibitors. The hit compounds(Diphenyleneiodonium chloride) were tested for cytotoxicity against Vero cells to determine the selectivity index, and time-kill kinetics were determined against Mycobacterium fortuitum. The compound's ability to synergize with amikacin, ceftriaxone, ceftazidime and meropenem was determined using fractional inhibitory concentration indexes followed by its ability to decimate mycobacterial infections ex vivo.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TRPVChannel, TRPChannel, TRPV Channel, TRP Channel, Transient receptor potential channels, Reactive Oxygen Species, ReactiveOxygenSpecies, Inhibitor, inhibit, NOX, NOS, NADPH Oxidase, DPI, Diphenyleneiodonium, Diphenyleneiodonium Chloride, Diphenyleneiodonium chloride

Compound Identifiers

PubChem CID

2733504