Dihydrofluorescein diacetate

SKU: orb1307199

Description

Dihydrofluorescein diacetate (DCFH-DA) is a cell-permeable, non-fluorescent probe used to detect reactive oxygen species (ROS) in both live cells and cell-free biochemical assays. It is widely applied in oxidative stress research within fields such as neurobiology, toxicology, and pharmacology for in vitro and in vivo studies.

Key Properties

• CAS Number: 35340-49-9
• MW: 418.4
• Purity: 99.98% (May vary between batches)
• Formula: C₂₄H₁₈O₇
• SMILES: CC(=O)Oc1ccc2C(c3ccc(OC(C)=O)cc3Oc2c1)c1ccccc1C(O)=O
• Target: Others
• Solubility: DMSO:100 mg/mL (239.01 mM)

Bioactivity

• In Vitro:
  Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence sharply delineates filaments that correspond in all detail to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix. Dihydrofluorescein diacetate may be a useful quantitative method to measure the oxidation potential of nanoparticle-treated cells. Dihydrofluorescein diacetate shows fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium.
• Cell Research:
  Dihydrofluorescein diacetate to measure intracellular ROS levels Operation steps 1. Cell culture: Cultivate cells in a normal way until they are in a suitable state. 2. Dye treatment: Add 20 μM DHF for 30 minutes. 3. Rinse with PBS to remove residual dye. 4. Observe the fluorescence intensity under a microscope to determine the ROS level in mitochondria.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Diacetyldihydrofluorescein, Dihydrofluorescein, Dihydrofluorescein diacetate, Dihydrofluorescein Diacetate, Inhibitor, inhibit

Compound Identifiers

PubChem CID

629056