Dihydroethidium

SKU: orb1306723

Description

Dihydroethidium is a cell-permeable fluorescent probe used to detect reactive oxygen species (ROS) such as superoxide. It exhibits blue cytoplasmic fluorescence (Ex/Em 518/616 nm) that diminishes upon oxidation. This indicator is widely applied in both in vitro and in vivo studies of oxidative stress in fields like neuroscience and cardiovascular research.

Key Properties

• CAS Number: 104821-25-2
• MW: 315.41
• Purity: 99.91% (May vary between batches)
• Formula: C₂₁H₂₁N₃
• SMILES: CCN1C(c2ccccc2)c2cc(N)ccc2-c2ccc(N)cc12
• Target: Others
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:0.5 mg/mL (1.59 mM);DMSO:125 mg/mL (396.31 mM)

Bioactivity

• In Vitro:
  METHODS: Detection of cellular peroxides by Dihydroethidium: 1. Prepare a 5-10 mM stock solution of Dihydroethidium dye in DMSO, protect from light, and store in portions at -20℃. Dilute the stock solution with physiological buffers (e.g., PBS, HBSS, HEPES) to obtain a working solution of 5-20 µM Dihydroethidium dye. The optimal working concentration must be determined empirically. 3. Add the Dye Working Solution to the cells at a 1:1 ratio (100 µL of Dye Working Solution to 100 µL of Cell Suspension) and incubate for 5-60 minutes at room temperature or 37℃. 4. Wash with PBS and then perform fluorescence microscopy or flow cytometry. Dihydroethidium itself is blue fluorescent with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm, and after dehydrogenation, it binds to RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm, and 518 nm can be used as the excitation wavelength in practice.
• Cell Research:
  Dihydroethidium detection of cell peroxides 1. Prepare 5-10 mM Dihydroethidium dye stock solution with DMSO, protect from light, and store in aliquots at -20°C. 2. Dilute the stock solution with physiological buffer (such as PBS, HBSS, HEPES) to obtain 5-20 µM Dihydroethidium dye working solution. The optimal working concentration must be determined empirically. 3. Add the dye working solution to the cells at a ratio of 1:1 (100 µL cell suspension to 100 µL dye working solution) and incubate at room temperature or 37°C for 5-60 minutes. 4. After washing with PBS, perform fluorescence microscopy or flow cytometry detection. Dihydroethidium itself is a blue fluorescent substance with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm. After dehydrogenation, it combines with RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm. In actual observation, 518 nm can also be used as the excitation wavelength.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dihydroethidium, Inhibitor, Hydroethidine, inhibit, PD-MY 003, PD-MY003, PD-MY-003

Compound Identifiers

PubChem CID

128682