DGAT1 Antibody

SKU: orb1247610

Description

DGAT1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 55kDa band observed in Rat Duodenum lysates (calculated MW of 55.3kDa according to NP_036211.1). Recommended concentration: 0.3-1.0µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QNSMKPFKDMDYS
• Target: DGAT1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DGAT1, diacylglycerol O-acyltransferase homolog 1 (mouse) , ARGP1, DGAT , ACAT related gene product 1, acyl coenzyme A:cholesterol acyltransferase related gene 1, diacylglycerol O-acyltransferase 1, diglyceride acyltransferase

orb1247610 (0.3 ug/ml) staining of Rat Duodenum lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247610 (3.75 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

DGAT1

UniProt

O75907

RefSeq (Protein):NP_036211.1