DSG3 Antibody

SKU: orb1252262

Description

DSG3 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Application Notes:
  WB: 0.5-1 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RTThe stated application concentrations are suggested starting amounts. Titration of the Desmoglein 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: DSG3/1535
• Immunogen: A protein fragment corresponding to the extracellular portion of human DSG3 was used as the immunogen for the Desmoglein 3 antibody.
• Target: DSG3
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human skin with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human lung squamos cell carcinoma with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

Quick Database Links

Gene Symbol

DSG3

UniProt

P32926