Desmoglein 3 Antibody [DSG3/1535]

• Catalog Number: orb1252262
• Category: Antibodies
• Description: Desmoglein 3 Antibody [DSG3/1535]
• Target: DSG3
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.2 mg/mL
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Immunogen: A protein fragment corresponding to the extracellular portion of human DSG3 was used as the immunogen for the Desmoglein 3 antibody.
• UniProt ID: P32926
• Tested applications: FC, IF, IHC-P, WB
• Application notes: WB: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTThe stated application concentrations are suggested starting amounts. Titration of the Desmoglein 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Antibody Type: Primary Antibody
• Clone Number: DSG3/1535
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

IHC testing of FFPE human skin with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

IHC testing of FFPE human lung squamos cell carcinoma with Desmoglein 3 antibody (clone DSG3/1535). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.