DSG1 Antibody

SKU: orb1249466

Description

DSG1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in lysates of cell lines A431, HeLa, K562 and MOLT4 gave no specific signal but low background (at antibody concentration up to 1µg/mL).Immunohistochemistry:In paraffin embedded Human Skin showsstaining of the cellular membranes of keratinocytes. Recommended concentration, 4-6µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-YTTSDTLKPSVHVHD
• Target: DSG1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DSG1, desmoglein 1, CDHF4, DG1, DSG, desmosomal glycoprotein 1, pemphigus foliaceus antigen

orb1249466 (3.8 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

DSG1

UniProt

Q02413

RefSeq (Protein):NP_001933.2