DEPC

SKU: orb1944980

Description

Diethyl pyrocarbonate (DEPC) is an irreversible RNase inhibitor that functions by carboxyethylating histidine residues, inactivating enzymes. It is widely used in molecular biology for in vitro RNA handling and has been applied in vivo to study chemosensitivity, such as in rabbit models.

Key Properties

• CAS Number: 1609-47-8
• MW: 162.14
• Purity: 99.58% (May vary between batches)
• Formula: C₆H₁₀O₅
• SMILES: CCOC(=O)OC(=O)OCC
• Target: Others
• Solubility: DMSO:55 mg/mL (339.21 mM);H2O:30 mg/mL (185.03 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:5 mg/mL (30.84 mM)

Bioactivity

• In Vivo:
  Insulin binding to rat liver plasma membranes is inhibited in a time- and dose-dependent fashion by prior treatment of membranes with the histidine-specific reagent diethyl pyrocarbonate.
  Read more...

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Diethyl pyrocarbonate, DEPC, RNase

Compound Identifiers

PubChem CID

3051