Decorin antibody

SKU: orb2276298

Description

Decorin antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Fallopian tube: A strong staining should be seen in the stroma while epithelial cells remain negative. Negative Control: Fallopian tube: Epithelial cells should not show any staining. Cellular Localization: Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: Biorbyt-537R
• Immunogen: Recombinant human Decorin protein fragment (around aa212-336) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Bone proteoglycan II; CSCD; Dermatan sulphate proteoglycans II (DSPG2); PG40; PGII; PGS2; Proteoglycan core protein; SLRR1B; Small leucine rich protein 1B

Fallopian tube mucosa Strong decorin immunostaining in the stroma while staining is completely lacking in epithelial cells decorin immunohistochemistry.

Serous high grade carcinoma of the ovary showing decorin staining of the stroma.

Strong decorin staining of the stroma while urothelium remains unstained in the urinary bladder.

Quick Database Links

UniProt

P07585