DBR1 Recombinant Mouse Monoclonal Antibody

• Catalog Number: orb1172803
• Category: Antibodies
• Description: DBR1 Recombinant Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: C11A2
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2b/Kappa
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:200-1:1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=0
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DBR1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 4°C overnight, Lysate: 1: Rat heart, 2: Mouse heart, 3: HeLa, 4: A431, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 75 kDa, Observed MW: 75 kDa.

Tissue: Mouse brain, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1172803.

Tissue: Rat brain, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1172803.