Dacarbazine

SKU: orb1309703

Description

Dacarbazine (DTIC-Dome) is a chemotherapeutic agent with established activity against malignant melanoma. It is widely used in both in vitro cytotoxicity assays and in vivo tumor model studies to investigate its alkylating mechanism and therapeutic potential in oncology research.

Research Area

Cell Biology, Molecular Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 4342-03-4
• MW: 182.18
• Purity: 99.00% (May vary between batches)
• Formula: C₆H₁₀N₆O
• SMILES: N(=NN(C)C)C1=C(C(N)=O)N=CN1
• Target: DNA/RNA Synthesis,Apoptosis,Nucleoside Antimetabolite/Analog
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:3 mg/mL (16.47 mM)

Bioactivity

• In Vivo:
  Treatment of combination of Axitinib and DTIC demonstrates significant antitumor activity against melanoma flank xenografts, reduces tumor cell proliferation and decreases the area of tumor necrosis and increases apoptosis. It also reduces meta-tasis-related factors and prolongs lifespan in mice.
• In Vitro:
  Treatment of Dacarbazine sensitizes melanomas to lysis of peptide-specific CTL and it is is mediated through Fas-independent pathway.
• Cell Research:
  Apotosis assay: UACC903 (UACC) cells treated with or without DTIC (20 μM) for 48 h are incubated with agonist anti-Fas Ab, CH-11 (500 ng/ml), for 5 and 16 h, respectively. To distinguish whether death/apoptosis is mediated by FasR, blocking anti-Fas Ab ZB4 (2 mg/ml) is added in the corresponding groups. At the end of incubation, cells are harvested and stained with propidium iodide and FITC-annexin V (BD PharMingen) for detecting apoptosis according to the instruction provided by the company. Ten thousand cells from each group are collected, and dead and apoptotic cells are analyzed without gating using CellQuest software. (Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RNASynthesis, RNA Synthesis, T cell, inhibit, Nucleoside Antimetabolite, Nucleoside Antimetabolite/Analog, NucleosideAntimetabolite, metastatic malignant melanoma, Inhibitor, Imidazole Carboxamide, Apoptosis, antineoplastic, Analog, B cell, Dacarbazine, DTIC-Dome, DNA Synthesis, DNA polymerase beta, DNASynthesis

Compound Identifiers

PubChem CID

2942