D159687

SKU: orb1301761

Description

D159687 is a selective phosphodiesterase 4D (PDE4D) inhibitor. It demonstrates a procognitive effect, enhancing memory in rodent novel object recognition tests, but lacks antidepressant or anxiolytic activity in standard models. This profile makes it a useful research tool for studying PDE4D's specific role in cognitive processes.

Research Area

Metabolism Research

Key Properties

• CAS Number: 1155877-97-6
• MW: 366.84
• Purity: 97.57% (May vary between batches)
• Formula: C₂₁H₁₉ClN₂O₂
• SMILES: COc1ccc(Cc2ccc(NC(N)=O)cc2)cc1-c1cccc(Cl)c1
• Target: PDE
• Solubility: DMSO:150 mg/mL (408.9 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (10.9 mM)

Bioactivity

• In Vitro:
  D159687 induced a transient increase in CREB phosphorylation which peaked at 6 hrs after treatment ( F (3, 20) = 3.731, P = 0.0425). CREB phosphorylation was optimal at 1 μM ( F (3, 20) = 4.194, P = 0.0302).
• Cell Research:
  cAMP concentrations were measured using the enzyme-linked immunosorbent assay. On the day of treatment, the attached HT-22 cells were washed with warm phosphate buffer saline and then incubated for 10 min with PBS supplemented with various concentrations of D159687 and further for 10 min with 10 nM isoproterenol in the system52. After incubation, PBS containing the drugs was removed and the plates were let dry roughly before 200 μl HCl (0.1 M) was added to each well to lyse the cells. Cell lysates were collected into Eppendorf tubes and centrifuged at 800 g for 15 min. Supernatants were collected for immediate assay or stored frozen for assay later using the cAMP complete ELISA kit according to the assay protocol.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

D159687, D-159687, D 159687, inhibit, Inhibitor, PDE4D, Phosphodiesterase (PDE), phosphodiesterase

Compound Identifiers

PubChem CID

57703712