Cytokeratin 10 antibody

SKU: orb2276486

Description

Cytokeratin 10 antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining. Negative Control: Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: MSVA-610M
• Immunogen: Recombinant full-lenth human KRT10 protein.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT12

Absence of KRT10 staining in non keratinizing squamous epithelium of the esophagus.

KRT10 is strongly positive in all suprabasal cell layers of the epidermis and in associated sebaceous glands and hair follicles. The basal cell layer remains strictly KRT10 negative.

Strong Cytokeratin 10 immunostaining in a squamous cell carcinoma of the vulva. The basal cell layers are largely unstained.

Quick Database Links

UniProt

P13645