Cynomolgus Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His Tag&Tag Free (MALS verified)

SKU: orb3158174

Description

Integrin alpha 5 beta 1 / Integrin α5β1, which is a major cellular receptor for the extracellular matrix protein fibronectin and plays a fundamental role during mammalian development., is composed of α5 (ITGA5/CD49e) and β1 (ITGB1/CD29) subunits. Integrins are adhesion receptors that transmit bidirectional signals across the plasma membrane. The Integrin alpha 5 beta 1 and its primary extracellular matrix ligand fibronectin (Fn) are of great biological importance.

Research Area

Immunology & Inflammation

Images & Validation

• Application Notes:
  Cynomolgus ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 111.2 kDa (ITGA5) & 83.6 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-160 kDa (ITGA5) and 95-110 kDa (ITGB1) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (NR) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: Integrin alpha 5 beta 1
• Reactivity: Monkey
• Tag: C-10×His | Native
• Molecular Weight: 111.2 kDa (ITGA5) & 83.6 kDa (ITGB1)
• Expression Region: Phe 42 - Tyr 999 (ITGA5) & Gln 161 - Asp 868 (ITGB1)
• Purity: 95%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: 50 mM HEPES, 150 mM NaCl, pH 7.5
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Integrin alpha 5 beta 1

RefSeq:A0A7N9D0D7, ITGB1, ITGA5, XP_005571144.3