Cyclovirobuxin D

SKU: orb1223832

Description

Extracted from Buxus microphylla;Suitability:Chloroform,methanol and ethanol;Store the product in sealed,cool and dry condition.

Key Properties

• CAS Number: 860-79-7
• MW: 402.66
• Purity: >98% (HPLC)
• Formula: C₂₆H₄₆N₂O
• SMILES: C[C@H](NC)[C@H]1[C@H](O)C[C@@]2(C)[C@]3([H])CC[C@@]4([H])C(C)(C)[C@@H](NC)CC[C@]4(C5)[C@@]35CC[C@]12C
• Target: Others
• Solubility: Ethanol: 20 mg/mL (49.66 mM)

Bioactivity

• In Vitro:
  Cyclovirobuxine D (0-240 μM; 24-72 hours) shows a concentration- and time-dependent reduced cell viability after CVB-D treatment, only 10% MGC-803 cells and 20% MKN28 cells are alive at 72 h after treatment with 240 μM. Cyclovirobuxine D (0-120 μM; 48 hours) arrests the cell cycle of gastric cancer cells at S phase in a concentration-dependent manner. Cyclovirobuxine D (0-120 μM; 48 hours) leads to apoptosis in gastric cancer cells in a concentration-dependent manner, especially early stage apoptosis. Cyclovirobuxine D (0-120 μM; 48 hours) causes apoptosis via up-regulation of the apoptosis- related proteins, cleaved Caspase-3 and ratio of Bax/Bcl-2, in gastric cancer cells. Cell Viability Assay Cell line: MGC-803 and MKN28 cells. Concentration: 0, 30, 60, 120 and 240 μM. Incubation time: 24, 48, 72 hours. Result: Reduced Cell Viability and Colony Formation Ability of Gastric Cancer Cells Cell Cycle Analysis Cell line: MGC-803 and MKN28 cells. Concentration: 0, 30, 60, and 120 μM. Incubation time: 48 hours. Result: Arrested cell cycle progressions of MGC-803 and MKN28 cells. Apoptosis Analysis Cell line: MGC-803 and MKN28 cells. Concentration: 0, 30, 60, and 120 μM. Incubation time: 48 hours. Result: Induced apoptosis of MGC-803 and MKN28 cells. Western blot analysis. Cell line: MGC-803 and MKN28 cells. Concentration: 0, 30, 60, and 120 μM. Incubation time: 48 hours. Result: Up-regulated cleaved Caspase-3 and Bax and decreased the expression of Bcl-2 expression.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CVB-D | NSC 91722

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