Cyclophosphamide hydrate

SKU: orb1310365

Description

Cyclophosphamide hydrate

Research Area

Immunology & Inflammation, Molecular Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 6055-19-2
• MW: 279.10
• Purity: ≥95% (May vary between batches)
• Formula: C₇H₁₇Cl₂N₂O₂P·H₂O
• SMILES: O.ClCCN(CCCl)P1(=O)NCCCO1
• Target: DNA,DNA Alkylator/Crosslinker,MRP
• Solubility: DMSO:250 mg/mL (895.74 mM);H2O:7 mg/mL (25.08 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (17.91 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  RAW 264.7 cells:145.44 μg/mL|HepG2 cells:4.81 μg/mL|HeLa cells:3.63 μg/mL|AChE:511 μM|HEK293 cells:> 100 μg/mL
• In Vivo:
  METHODS: To test the antitumor activity in vivo, Cyclophosphamide hydrate (50-150 mg/kg) was administered as a single intraperitoneal injection to BALB/c mice bearing mouse colorectal tumor CT26. RESULTS: Cyclophosphamide hydrate induced a significant reduction in tumor volume. METHODS: Cyclophosphamide hydrate (140 mg/kg) was administered orally to Balb/c mice bearing mouse mammary carcinoma tumor 4T1 every six days for eighteen days to determine the antitumor activity in vivo. RESULTS: Cyclophosphamide hydrate treatment significantly inhibited tumor growth in mice.
• In Vitro:
  METHODS: Macrophage Raw 264.7 was treated with Cyclophosphamide hydrate (10-250 μg/mL) for 48 h. Cell viability was measured by MTT assay. RESULTS: Cyclophosphamide hydrate was cytotoxic to Raw 264.7 with an IC50 of 145.44 μg/mL. METHODS: Human breast cancer cells MDA-MB-231 and MDA-MB-435S were treated with Cyclophosphamide hydrate (0.25-1 mM) for 4-24 h, and cell migration was detected by Wound healing assay. RESULTS: The increase in the number of migrating MDA-MB-231 cells was dependent on the concentration of Cyclophosphamide, while migration to MDA-MB-435S cells was significantly reduced and independent of Cyclophosphamide concentration.
• Cell Research:
  9L/pBabe, 9L/Bax, and 9L/Bcl-2 cells are treated with 12, 24, or 50 μM MFA for 72 h. Cells remaining on the plates at 0, 24, 48, and 72 h are washed twice with cold PBS and then stained for 5 min with crystal violet [1.25 g of crystal violet dissolved in a solution containing 50 mL of 37% formaldehyde and 450 mL of methanol]. The stained cells are washed three times in tap water and the plates are allowed to dry. The stain is eluted from the cells with 70% ethanol and the absorbance is then read at 595 nm. The staining intensity of each drug-treated sample (A 595) is then graphed as a percentage of the staining intensity at the 0-h time point.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Crosslinker, Cyclophosphamide, Cyclophosphamide hydrate, Cyclophosphamide Hydrate, Cyclophosphamide Monohydrate, Cyclophosphamide monohydrate, DNA Alkylator, DNA Alkylator/Crosslinker, DNA, DNAAlkylator, Inhibitor, MRP1, inhibit

Compound Identifiers

PubChem CID

22420