Cyanine5.5 maleimide chloride

SKU: orb2566545

Description

Cyanine5.5 maleimide chloride is a near-infrared fluorescent dye used to label proteins, antibodies, and small molecules via thiol-maleimide chemistry. Its favorable photophysical properties enable sensitive in vivo imaging and in vitro detection applications in bioimaging and analytical research.

Key Properties

• CAS Number: 2409591-45-1
• MW: 741.36
• Purity: 99.93%
• Formula: C₄₆H₄₉ClN₄O₃
• SMILES: [Cl-].O=C1C=CC(=O)N1CCNC(=O)CCCCCN2C=3C=CC=4C=CC=CC4C3C(C2=CC=CC=CC5=[N+](C=6C=CC=7C=CC=CC7C6C5(C)C)C)(C)C
• Target: Others
• Solubility: DMSO:40 mg/mL (53.95 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:1.5 mg/mL (2.02 mM)

Bioactivity

• In Vitro:
  Cyanine5.5 maleimide chloride is a conjugated dye composed of the Cyanine5.5 fluorophore and a maleimide reactive group. Cyanine 5.5 is a near-infrared fluorescent cyanine dye widely used for in vivo small animal imaging. It can be employed to label proteins, antibodies, peptides, and nanoparticles. Maleimide is a commonly used group in bioconjugation reactions, capable of forming stable thioester linkages with sulfhydryl groups (-SH) through Michael addition, thereby achieving labeling. Although amino groups (such as the side chains of lysine and arginine) also exhibit reactivity, under neutral or slightly acidic buffer conditions, maleimide demonstrates significantly higher selectivity toward sulfhydryl groups (reacting over 1000 times faster than with amino groups), enabling rapid, highly selective, and high-yield labeling. Furthermore, sulfhydryl groups are widely present as functional groups in biomolecules, such as cysteine in proteins and disulfide bonds. Consequently, maleimide/thiol labeling has become the second most commonly used bioconjugation method after NHS/amine labeling.

Storage & Handling

• Storage: keep away from direct sunlight | store at -20°C | Shipping with blue ice.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cy5.5 maleimide chloride

Compound Identifiers

PubChem CID

155889078