Cyanine5.5 amine

SKU: orb2566546

Description

Cyanine5.5 amine is an amino-reactive near-infrared (NIR) dye (Ex/Em 648/710 nm) with minimal background fluorescence. It is widely used for labeling biomolecules in applications such as in vivo imaging, fluorescence microscopy, and molecular sensing due to its deep tissue penetration.

Key Properties

• CAS Number: 2097714-45-7
• MW: 753.88
• Purity: 99.60%
• Formula: C₄₆H₅₈Cl₂N₄O
• SMILES: CC1(C)C2=C(N(CCCCCC(NCCCCCCN)=O)C1=CC=CC=CC=3C(C)(C)C4=C([N+]3C)C=CC=5C4=CC=CC5)C=CC=6C2=CC=CC6.[Cl-].Cl
• Target: Others
• Solubility: DMSO:80 mg/mL (106.12 mM)

Bioactivity

• In Vivo:
  Instructions: Real-time monitoring of Cyanine5.5 amine-labeled nanoparticles (Cy5.5-PLGA) in retinal blood vessels was conducted as follows: Freshly prepared Cy5.5-PLGA suspensions (resuspended in 0.5 ml 1% poloxamer 188, gently vortexed, and left for 30 min at ambient temperature before use) were administered intravenously (0.5 mL). Rats were anesthetized, their eyes treated with Neosynephrine-POS 5%, and Vidisic eye gel was applied. The rats were positioned under a confocal scanning microscope with a cannula in the tail vein. Fluorescence in the retina was observed and imaged at different time points (0, 1, 3, 5, 15, 30, 60, 90 min) while the rats were kept on a heating plate. After imaging, the rats were euthanized with an overdose of anesthetic, their eyeballs enucleated, and placed in cooled HEPES-buffered solution (135 mM NaCl, 5 mM NaOH, 2.5 mM KCl, 7 mM MgCl2, 10 mM HEPES, 10 mM glucose; pH 7.4). The anterior segment and vitreous body were removed, and the retina was carefully separated and flattened on a culture plate. Retinal whole mounts were incubated with 0.1 mg/mL Hoechst 33342 in HEPES solution for 20 min for nuclei staining, fixed with 4% paraformaldehyde for 20 min, washed with HEPES solution, and imaged immediately with a microscope.

Storage & Handling

• Storage: keep away from direct sunlight | store at -20°C | Shipping with blue ice.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

(E)-6-O-Coumaroylscandoside methyl ester

Compound Identifiers

PubChem CID

172878591