CUL4B Antibody

SKU: orb1257511

Description

CUL4B Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CUL4B (NP_003579.3).
• Target: CUL4B
• Molecular Weight: Observed: 115kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Cullin-4B, CUL-4B, CUL4B, KIAA0695

Western blot analysis of extracts of various cell lines, using CUL4B antibody (orb1257511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat liver using CUL4B antibody (orb1257511) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using CUL4B antibody (orb1257511) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse pancreas using CUL4B antibody (orb1257511) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using CUL4B antibody (orb1257511) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of U2OS cells using CUL4B antibody (orb1257511). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.

Quick Database Links

Gene Symbol

CUL4B

UniProt

Q13620