Costunolide

SKU: orb1304867

Description

Costunolide is a sesquiterpene lactone with documented anti-inflammatory, antioxidant, and pro-apoptotic activities. It is widely used in pharmacological research, with applications in studying cancer, inflammation, and oxidative stress in both cellular and animal models.

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 553-21-9
• MW: 232.32
• Purity: >99.99% (May vary between batches)
• Formula: C₁₅H₂₀O₂
• SMILES: [H][C@]12OC(=O)C(=C)[C@]1([H])CC\C(C)=C\CC\C(C)=C\2
• Target: Apoptosis,Telomerase,Endogenous Metabolite
• Solubility: DMSO:240 mg/mL (1033.06 mM);Ethanol:11.6 mg/mL (49.93 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (21.52 mM)

Bioactivity

• Target IC50:
  FPTase:20 μM|Telomerase (MDA-MB-231 cells):65 μM|Telomerase (MCF-7 cells):90 μM
• In Vivo:
  Costunolide has been shown to inhibit cell proliferation in a dose-dependent manner over a 48-hour treatment in various human tumor cells, including A549, SK-OV-3, SK-MEL-2, XF498, and HCT-15. It induces apoptosis in HL-60 human leukemia cells through ROS-mediated mitochondrial permeability transition and cytochrome C release. Additionally, Costunolide exhibits significant antifungal activity against organisms such as Trichophyton rubrum, T. mentagrophytes, and Microsporum gypseum. The compound also suppresses telomerase activity and cell proliferation in MCF-7 and MDA-MB-231 cells in a concentration and time-dependent manner. Furthermore, Costunolide dose-dependently inhibits the farnesylation of lamin B by farnesyl protein transferase, demonstrating its diverse biochemical impacts.
• In Vitro:
  Costunolide reduces neovascularization induced by ethyl ester resin scale plaster in the corneal micropocket model of mice. It inhibits angiogenesis by blocking the vascular endothelial growth factor signaling pathway.
• Cell Research:
  1) Plate 500-10,000 cells in 200 μL media per well in a 96 well plate. Leave 8 wells empty for blank controls. 2) Incubate (37 °C, 5% CO2) overnight to allow the cells to attach to the wells. 3) Add 2 μL of Costunolide dissolved in DMSO to each well. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the samples into the media. 5) Incubate (37 °C, 5% CO2) for 48 hours to allow Costunolide to take effect. 6) Make 2 mL or more of MTT solution per 96 well plate at 5 mg/mL in PBS. Do not make a stock as MTT in solution is not stable long-term. 7) Add 20 μL MTT solution to each well. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the MTT into the media. 8) Incubate (37 °C, 5% CO2) for 1-5 hours to allow the MTT to be metabolized. 9) Dump off the media. (Dry plate on paper towels to remove residue if necessary. 10) Resuspend formazan (MTT metabolic product) in 200 μL DMSO. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the formazan into the solvent. 11) Read optical density at 560 nm and subtract background at 670 nm. Optical density should be directly correlated with cell quantity. (Only for Reference)

Storage & Handling

• Storage: store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

regenerating, promoting, Telomerase (MCF-7), Telomerase (MDA-MB-231), NSC106404, NSC-106404, NSC 106404, EndogenousMetabolite, Endogenous Metabolite, FPTase, hair, Costunolide, Costus lactone, (+)-Costunolide, Apoptosis, anti-allergic, antidiabetic, antioxidants

Compound Identifiers

PubChem CID

133640194