Cortisol EIA Kit

SKU: orb1822515

Description

Quantitative colorimetric detection of cortisol

Research Area

Cancer Biology, Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Application Notes:
  The Cortisol EIA kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and tissue culture media samples. This kit measures total cortisol in extracted samples and in serum and plasma and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. After an 1 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the cortisol in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Key Properties

• Reactivity: All
• Sample Types: Dried Fecal Samples, Saliva, Urine, Serum, EDTA Plasma, Heparin Plasma, Tissue Culture Media
• Assay Type: Competitive
• Range: 100 - 3200 pg/ml
• Sensitivity: 17.3 pg/ml

Storage & Handling

• Storage: 4°C
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

(11β)-11,17,21-trihydroxypregn-4-ene-3,20-dione

Typical Standard Curve for the Cortisol EIA Kit (Enzyme Immunoassay) Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 100 - 3200 pg/ml.

Linearity was determined by taking two human urine samples diluted 1:140, one with a low diluted cortisol level of 163.9 pg/mL and one with a higher diluted level of 2, 974.9 pg/mL and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.

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Cortisol EIA Kit