Corticosterone

SKU: orb1310140

Description

Corticosterone

Research Area

Metabolism Research, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 50-22-6
• MW: 346.46
• Purity: 99.82%
• Formula: C₂₁H₃₀O₄
• SMILES: C[C@]12C[C@H](O)[C@H]3[C@@H](CCC4=CC(=O)CC[C@]34C)[C@@H]1CC[C@@H]2C(=O)CO
• Target: Endogenous Metabolite,iGluR,Glucocorticoid Receptor
• Solubility: H2O:insoluble;10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.47 mg/mL (10.02 mM);Ethanol:3.5 mg/mL (10.1 mM);DMSO:240 mg/mL (692.72 mM)

Bioactivity

• Target IC50:
  OCT3 (human):0.2 µM|OCT2 (rat):4 µM|OCT1 (rat):150 µM|OCT2 (human):30 µM|OCT1 (human):10 µM
• In Vivo:
  METHODS: To investigat the effects on depressive-like behavior in mice, corticosterone (20 mg/kg, suspended in physiological saline containing 0.1% DMSO and 0.1% Tween-80) was injected Subcutaneously into C57BL/6N mice once daily for 1-5 weeks. Results: Repeated injections of corticosterone increased immobility behavior in a time-dependent manner in the forced swimming and tail suspension tests. A the same time, this injection pattern had a time-dependent effect on tyrosine hydroxylase levels in the mouse hippocampus. These RESULTS are consistent with correlations in models of stress-induced depression. METHODS: To investigat the modulatory effects on fear, Corticosterone (2 mg/kg, 2.5% EtOH in saline) was injected intraperitoneally into C57B-/- mice trained in an auditory fear conditioned reflex paradigm. Results: Corticosterone affected memory consolidation and recovery; in Male mice, corticosterone consistently increased freezing behavior to tones, whereas in f Male mice, corticosterone reduced freezing behavior 24 h after training.
• In Vitro:
  METHODS the mouse mammary hyperplastic epithelial cell line TM10 was treated with Corticosterone (50-200 µM) for 24-72 h the cell number was detected by crystal violet staining. Results: Corticosterone significantly inhibited cell growth by 50, 57, and 76% after treatment with 50, 100, and 200 µM for 72 hours. METHODS: Human neuroblastoma cells SK-N-BE(2)C were treated with Corticosterone (1-100 nM) for 1-14 days, and gene expression levels were measured by RT-PCR. Results: Corticosterone increased NET mRNA levels in SK-N-BE(2)C cells.
• Cell Research:
  HEK293 cells are grown in 6-cm dishes in 10% fetal bovine serum DMEM medium. When cells are 90% confluent the medium is changed to 0.5% fetal bovine serum DMEM to limit serum-induced up-regulation of SGK. For in vitro phosphorylation analysis the following approach is used. HEK293 cells are transfected with or without SGK1 s All interfering RNA. One day after transfection, cells are treated without or with 100 nM corticosterone for 30 min, washed, and maintained in 0.5% fetal bovine serum DMEM for 1.5 h. Then cells are lysed in the CytoBuster protein extraction reagent containing protease inhibitors. Cell lysates are centrifuged at 16,000 × g at 4 °C for 20 min the supernatants (40 μl, ～50 μg of total protein) are incubated with 1 μg of purified GST fusion protein of wild-type GDI or its mutants for 30 min at 30 °C in the reaction buffer (30 mM HEPES, pH 7.5, 10 mM MgCl2, 30 μM ATP, 1 μCi of [γ-32P]ATP, 100 nM calyculin, 1 μM okadaic acid). SDS-PAGE is carried out, and phosphorylated GDI is visualized with autoradiography.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

CD40, Corticosterone, Corticosterone (From plants), 11beta,21-Dihydroxyprogesterone, 11b,21-Dihydroxyprogesterone, 17-Deoxycortisol, 11β,21-Dihydroxyprogesterone, BDNF, BMDC, B7.2, B7.1, MHC class II, Kendall's compound B, GlucocorticoidReceptor, Glucocorticoid Receptor, ethanol intake, EndogenousMetabolite, Endogenous Metabolite

Compound Identifiers

PubChem CID

5753