Connexin 40 Antibody (N-term)

• Catalog Number: orb1935260
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB01377
• Tested applications: IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:4000, IHC-P-Leica: 1:500
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 40380 Da
• Target: This Connexin 40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-134 amino acids from the N-terminal region of human Connexin 40.
• UniProt ID: P36382
• NCBI: NP_859054.1, NP_005257.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Gap junction alpha-5 protein, Connexin-40, Cx40, G
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of anti-GJA5 N-term Pab in Placenta lysate. GJA5 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemical analysis of paraffin-embedded human stomach tissue was performed on the Leica BOND RXm. Samples were incubated with primary antibody (1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-Connexin 40 Antibody (N-term) at 1:4000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.