ConA-Biotin Conjugated

SKU: orb2652830

Description

Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa; originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars α-mannosyl and α-glucosyl residues in glycoproteins.

Images & Validation

• Application Notes:
  Histochemistry 1: 250-1:1000; WB 1:500-1:2,500; ELISA 1:500-1:2,500. Dilute biotinylated lectin in PBS containing 0.1 mM calcium chloride ions. For Histochemistry the tissues are processed same as for Immunohistochemistry, after blocking step biotinylated lectin is applied followed by streptavidin conjugated to enzyme and chromogen. For WB incubate membrane with blocking protein followed by biotinylated lectin, streptavidin enzyme conjugate and chromogen. For ELISA the target proteins are absorbed on ELISA plate at a concentration of 5-10 µg/ml, followed by blocking with protein solution followed by Biotinylated lectin, Streptavidin enzyme conjugate, ELISA substrate. Please refer to Histochemistry, WB and ELISA protocol for detail information.

Storage & Handling

• Storage: 2-8 °C
• Buffer/Preservatives: 10 mM bicarbonate, 150 mM NaCl, pH 8, 0.1 mM Calcium chloride, 0.01mM manganese chloride and 0.05% sodium azide.
• Concentration: 5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only